Viral and Atypical Bacterial Respiratory Infections in a University Teaching Hospital.
Identifieur interne : 000627 ( Main/Exploration ); précédent : 000626; suivant : 000628Viral and Atypical Bacterial Respiratory Infections in a University Teaching Hospital.
Auteurs : Agca Harun ; Ener BeyzaSource :
- Japanese journal of infectious diseases [ 1884-2836 ] ; 2019.
Descripteurs français
- KwdFr :
- Adolescent, Adulte, Adulte d'âge moyen, Bactéries (), Bactéries (isolement et purification), Enfant, Enfant d'âge préscolaire, Femelle, Humains, Hôpitaux d'enseignement, Hôpitaux universitaires, Infections bactériennes (microbiologie), Infections bactériennes (épidémiologie), Infections de l'appareil respiratoire (microbiologie), Infections de l'appareil respiratoire (virologie), Infections de l'appareil respiratoire (épidémiologie), Jeune adulte, Liquide de lavage bronchoalvéolaire (microbiologie), Liquide de lavage bronchoalvéolaire (virologie), Maladies virales (virologie), Maladies virales (épidémiologie), Mâle, Nourrisson, Nouveau-né, Partie nasale du pharynx (microbiologie), Partie nasale du pharynx (virologie), Prévalence, Réaction de polymérisation en chaîne, Sujet âgé, Sujet âgé de 80 ans ou plus, Turquie (épidémiologie), Virus (), Virus (isolement et purification), Études rétrospectives.
- MESH :
- isolement et purification : Bactéries, Virus.
- microbiologie : Infections bactériennes, Infections de l'appareil respiratoire, Liquide de lavage bronchoalvéolaire, Partie nasale du pharynx.
- virologie : Infections de l'appareil respiratoire, Liquide de lavage bronchoalvéolaire, Maladies virales, Partie nasale du pharynx.
- épidémiologie : Infections bactériennes, Infections de l'appareil respiratoire, Maladies virales, Turquie.
- Adolescent, Adulte, Adulte d'âge moyen, Bactéries, Enfant, Enfant d'âge préscolaire, Femelle, Humains, Hôpitaux d'enseignement, Hôpitaux universitaires, Jeune adulte, Mâle, Nourrisson, Nouveau-né, Prévalence, Réaction de polymérisation en chaîne, Sujet âgé, Sujet âgé de 80 ans ou plus, Virus, Études rétrospectives.
- Wicri :
- geographic : Turquie.
English descriptors
- KwdEn :
- Adolescent, Adult, Aged, Aged, 80 and over, Bacteria (classification), Bacteria (isolation & purification), Bacterial Infections (epidemiology), Bacterial Infections (microbiology), Bronchoalveolar Lavage Fluid (microbiology), Bronchoalveolar Lavage Fluid (virology), Child, Child, Preschool, Female, Hospitals, Teaching, Hospitals, University, Humans, Infant, Infant, Newborn, Male, Middle Aged, Nasopharynx (microbiology), Nasopharynx (virology), Polymerase Chain Reaction, Prevalence, Respiratory Tract Infections (epidemiology), Respiratory Tract Infections (microbiology), Respiratory Tract Infections (virology), Retrospective Studies, Turkey (epidemiology), Virus Diseases (epidemiology), Virus Diseases (virology), Viruses (classification), Viruses (isolation & purification), Young Adult.
- MESH :
- geographic , epidemiology : Turkey.
- classification : Bacteria, Viruses.
- epidemiology : Bacterial Infections, Respiratory Tract Infections, Virus Diseases.
- isolation & purification : Bacteria, Viruses.
- microbiology : Bacterial Infections, Bronchoalveolar Lavage Fluid, Nasopharynx, Respiratory Tract Infections.
- virology : Bronchoalveolar Lavage Fluid, Nasopharynx, Respiratory Tract Infections, Virus Diseases.
- Adolescent, Adult, Aged, Aged, 80 and over, Child, Child, Preschool, Female, Hospitals, Teaching, Hospitals, University, Humans, Infant, Infant, Newborn, Male, Middle Aged, Polymerase Chain Reaction, Prevalence, Retrospective Studies, Young Adult.
Abstract
Respiratory viral and atypical bacterial agents lead to infections in a large spectrum, from mild symptoms to respiratory failure. In the present study, we aimed to detect multiple viral and bacterial agents in the respiratory samples of inpatients by real-time polymerase chain reaction (RT-PCR). Nasopharyngeal swabs and broncho-alveolar lavage samples from inpatients with respiratory infection symptoms at the Uludag University Hospital between December 1, 2015 and March 31,2018 were investigated. DNA/RNA was extracted using the EZ1 Virus Mini Kit v2.0 (Qiagen, Belgium) with the EZ1 extraction device (Qiagen, Belgium). The R-GENE® RT-PCR (Biomerioux, France) kit was used to detect influenza A, influenza B, respiratory syncytial virus (RSV), human metapneumovirus, rhinovirus/enterovirus (RV/EV), adenovirus, human bocavirus (hBoV), corona virus, parainfluenza virus, Chlamydia pneumoniae/Mycoplasma pneumoniae, and Legionella pneumophila in Rotor-Gene Q (Qiagen, Belgium). Patients were aged between 0 and 90 years. Overall, 177 (56.9%) patients were men and 134 (43.1%) were women. A total of 311 samples were analyzed, of which 214 (68.8%) were positive. In total, 360 agents, including 338 viruses and 22 bacteria, were detected. The commonest agents were influenza A+B (n = 65, 18,1%), hBoV (n = 64, 17.8%), RV/EV (n = 56, 15.6%), and RSV (n = 47, 13.1%). Rapid diagnosis of viral infections by RT-PCR is important for the specific treatment of patients.
DOI: 10.7883/yoken.JJID.2018.510
PubMed: 31257239
Affiliations:
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Le document en format XML
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moyen</term><term>Bactéries</term><term>Enfant</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Humains</term><term>Hôpitaux d'enseignement</term><term>Hôpitaux universitaires</term><term>Jeune adulte</term><term>Mâle</term><term>Nourrisson</term><term>Nouveau-né</term><term>Prévalence</term><term>Réaction de polymérisation en chaîne</term><term>Sujet âgé</term><term>Sujet âgé de 80 ans ou plus</term><term>Virus</term><term>Études rétrospectives</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Turquie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Respiratory viral and atypical bacterial agents lead to infections in a large spectrum, from mild symptoms to respiratory failure. In the present study, we aimed to detect multiple viral and bacterial agents in the respiratory samples of inpatients by real-time polymerase chain reaction (RT-PCR). Nasopharyngeal swabs and broncho-alveolar lavage samples from inpatients with respiratory infection symptoms at the Uludag University Hospital between December 1, 2015 and March 31,2018 were investigated. DNA/RNA was extracted using the EZ1 Virus Mini Kit v2.0 (Qiagen, Belgium) with the EZ1 extraction device (Qiagen, Belgium). The R-GENE<sup>®</sup> RT-PCR (Biomerioux, France) kit was used to detect influenza A, influenza B, respiratory syncytial virus (RSV), human metapneumovirus, rhinovirus/enterovirus (RV/EV), adenovirus, human bocavirus (hBoV), corona virus, parainfluenza virus, Chlamydia pneumoniae/Mycoplasma pneumoniae, and Legionella pneumophila in Rotor-Gene Q (Qiagen, Belgium). Patients were aged between 0 and 90 years. Overall, 177 (56.9%) patients were men and 134 (43.1%) were women. A total of 311 samples were analyzed, of which 214 (68.8%) were positive. In total, 360 agents, including 338 viruses and 22 bacteria, were detected. The commonest agents were influenza A+B (n = 65, 18,1%), hBoV (n = 64, 17.8%), RV/EV (n = 56, 15.6%), and RSV (n = 47, 13.1%). Rapid diagnosis of viral infections by RT-PCR is important for the specific treatment of patients.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Beyza, Ener" sort="Beyza, Ener" uniqKey="Beyza E" first="Ener" last="Beyza">Ener Beyza</name><name sortKey="Harun, Agca" sort="Harun, Agca" uniqKey="Harun A" first="Agca" last="Harun">Agca Harun</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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